Journal: Cancer Immunology, Immunotherapy : CII
Article Title: Tissue factor targeted near-infrared photoimmunotherapy: a versatile therapeutic approach for malignancies
doi: 10.1007/s00262-024-03903-2
Figure Lengend Snippet: Delivery of tisotumab or tiso-IR700 and efficacy of in vivo TF-targeted NIR-PIT in the HPAF-II subcutaneous xenograft model. A , Tumors were harvested 24 h after injecting tiso-DIG or isotype-DIG into mice and the distribution in HPAF-II tumors was examined by multiplex immunohistochemistry ( n = 4; images, × 200; scale bar, 100 μm). DIG and pCK are shown in green and purple, respectively. tiso-DIG, digoxigenin (DIG)-conjugated tisotumab. B , In vivo fluorescence imaging of tiso-IR700. Representative fluorescence images at 700 nm in an HPAF-II tumor bearing mouse. All images were acquired at the indicated time points after injecting tiso-IR700 into mice. AU, arbitrary units. C , Quantitative analysis of target-to-background ratio after injecting tiso-IR700 ( n = 5). D , Treatment schedule. E , Representative 700-nm fluorescence images before and after NIR-PIT. F , Tumor volume curves ( n = 10–11; mean ± SEM; repeated measures two-way repeated measures ANOVA followed by Tukey's test); *, p < 0.05; **, p < 0.01; *** p < 0.001; ****, p < 0.0001 versus the Control group. G , Survival curves ( n = 10–11, log-rank test with Bonferroni correction); ***, p < 0.001
Article Snippet: Tisotumab, an anti-human TF recombinant monoclonal antibody (mAb), was purchased from Creative Biolabs Inc. (Shirley, NY, USA).
Techniques: In Vivo, Multiplex Assay, Immunohistochemistry, Fluorescence, Imaging, Control